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Spatial Analysis with Human Tissue Samples

Banner FFPE and frozen tissue samples for spatial analysis

FFPE and frozen tissue samples for spatial analysis

FFPE and frozen tissue samples for spatial analysis

 

Human tissue samples are widely being used in medical, scientific, and clinical research and are valuable resources for multiple applications. FFPE and frozen tissue samples allow scientists to conduct research on rare diseases, evaluate the efficacy of drugs or targeted therapies and discover new biomarkers. Recently, two new cutting-edge techniques became popular amongst the researchers, namely spatial transcriptomics and spatial multiplex phenotyping.

 

Insights into cutting-edge techniques for human tissue specimens 

 

Spatial transcriptomics allows researchers to study gene expression in combination with information about the tissue architecture or spatial organization, resulting in the creation of a detailed gene expression map for tissues. The fundamental idea is to retain spatial information while profiling gene expression in the tissue. Thin sections of tissue are mounted onto slides that contain an arrangement of spots or arrays, each with unique molecular barcodes or capture probes. The tissue sections on the slides are then treated to allow mRNA molecules to bind to the capture probes or barcodes on the array. Since each capture probe is designed to target a specific gene or set of genes, this approach enables the identification of mRNA transcripts corresponding to the genes expressed in different locations of the tissue by applying reverse transcription and preparing sequencing libraries. Consequently, the spatial information from each array can be combined with DNA sequencing, resulting in a spatial gene expression map of the tissue. 

 

Fuel your spatial discoveries with FFPE and frozen tissue samples

 

The other technique, called multiplex phenotyping, has recently gained attention due to a new combination approach, in which FFPE blocks can be used to perform spatial immunofluorescence phenotyping with multiple dyes. While traditional phenotyping methods, like flow cytometry, use single-directed labeled antibodies which can target only specific proteins one at a time, multiplex immunofluorescence phenotyping allows the simultaneous detection of several biomarkers within a single experiment due to the utilization of multiple fluorescently labeled antibodies with distinct fluorochromes. This allows researchers to visualize multiple targets simultaneously with just one panel of labelled antibodies. Upon fluorescence imaging, the obtained data can be further analysed using software for quantification to create a spatial map of biomarkers within the tissue.

     

 

Specifications in detail:

  • Matrix: FFPE Tissue, frozen tissue
  • Disease presentation: Primary/secondary tumor, NAT
  • Receptor status: Estrogen, progesterone, HER2
  • TNM staging

With over 13,000 human tissue samples in our catalogue, Central BioHub is the leading platform for researchers from all over the world to find FFPE and frozen human tissue samples that meet their needs within seconds. Find your samples, reserve and order online - fast, easy and compliant.

 

  • FFPE human tissue samples:

https://centralbiohub.de/biospecimens/tissue-bank/human-tissue-samples

 

  • Frozen tissue samples:

https://centralbiohub.de/biospecimens/tissue-bank/frozen-tissue

 

  • Complete tissue inventory:

https://centralbiohub.de/biospecimens/tissue-bank

 

Try our expert search with advanced filter options and explore our full online inventory. Laboratory test parameters, clinical diagnosis, histological data and many more options can be applied to ensure a convenient browsing experience of over 13,000 human tissue samples and will take your research studies to a new level.  Find human tissue samples for your next discovery by clicking on clinical diagnosis, icd-10-cm codes, and laboratory parameters.

 

Central BioHub - Order biospecimens online.

FFPE (Formalin-Fixed Paraffin-Embedded) are prepared by fixing fresh tissue in formaldehyde to preserve the cellular structures and proteins. The fixed tissue is then dehydrated, cleared, and embedded in paraffin wax. Afterwards, it will be cut into small slices and transferred to a coverslip for further analysis. Central BioHub is the biggest provider for FFPE samples worldwide, which are commonly used by researchers in histological and immunohistochemical tissue studies, genomic and transcriptomic analysis as well as investigations that aim to discover new biomarkers.
Frozen tissue samples can be obtained by freezing biological tissue at ultra-low temperatures usually below -80 °C. In this process, cellular integrity and molecular components can be maintained and tissue damage due to ice crystal formation minimized. Therefore, tissue samples from miscellaneous sources and time periods can be analysed retrospectively, which enables researchers to study disease progression, genetic mutations and gene expression patterns in terms of epidemiological and epigenetic questions.
Human tissue samples can be stored for several years and sometimes even decades in specialized cryogenic freezers. However, proper handling and storage of frozen tissue samples are crucial to maintain their quality over a long period of time. As a certified distributor of human biospecimen, suppliers and logistical partners of Central BioHub undergo quality checks and validations during their onboarding process to provide flawless shipping and high-quality samples for our customers at any time.
Besides the benefits of long-term storage and the preservation of cellular structures, FFPE and frozen tissue samples allow researchers to combine biochemical and genetic data acquired by biotechnological methods with the spatial information of human tissue samples. This approach offers the opportunity of mapping gene expression patterns and molecular structures with the tissue architecture itself, resulting in valuable and detailed insights that lead to an improved biological understanding or result in the discovery of new possible targets for biomedical treatment approaches.